FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein application recombinant DNA technology. It can be acclimated for affection chromatography, again acclimated to abstracted recombinant, overexpressed protein from wild-type protein bidding by the host organism. It can aswell be acclimated in the abreast of protein complexes with assorted subunits.
A FLAG-tag can be acclimated in abounding altered assays that crave acceptance by an antibody. If there is no antibiotic adjoin the advised protein, abacus a FLAG-tag to this protein allows one to chase the protein with an antibiotic adjoin the FLAG sequence. Examples are cellular localization studies by immunofluorescence or apprehension by SDS PAGE protein electrophoresis.
The peptide arrangement of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be acclimated in affiliation with added affection tags, for archetype a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be alloyed to the C-terminus or the N-terminus of a protein. Some commercially accessible antibodies (e.g., M1/4E11) admit the epitope alone if it is present at the N-terminus. However, added accessible antibodies (e.g., M2) are position-insensitive.
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